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The Biophysics of Organ Cryopreservation by Geoffrey Collins (auth.), David E. Pegg, Armand M. Karow Jr.

By Geoffrey Collins (auth.), David E. Pegg, Armand M. Karow Jr. (eds.)

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Matheny, Functional preservation of the mammalian kidney. V. 4 M) in kidneys (rabbit and dog) perfused at 37, 25 or 10 ° C followed by transplantation (dog), J. Surge Res. 27:93 (1979). 11. J. Bickis, K. J. D. Henderson, Permeation kinetics of glycerol and dimethyl sulfoxide in Novikoff hepatoma ascites cells, Cryobiology 4:1 (1967). 12. E. Huggins, The effect of varying concentrations of glycerol on the weight of isolated rabbit kidney slices, J. Physio1. (London) 145:54P (1959). 13. B. Barner, Mannitol as an osmotic antagonist to dimethyl sulfoxide, Cryobiology 1:292 (1965).

Jacobsen We have used a similar solution, the LIC solution designed by yourself, which does not contain adenine or glutathione and it worked beautifully for 48 hours of hypothermic storage. Why did you use adenine instead of adenosine? Adenine has been demonstrated to produce vascular spasm in the afferent arterioles, which accounts for the anuria which has been reported after transplantation of kidneys perfused with adenine. Fahy I wanted to avoid the platelet activation caused by adenosine, so I used adenine instead.

G. Downes, R. Hoffman, J. O. Belzer, Mechanism of action of washout solutions for kidney preservation, Transplantation 16:46 (1973). 37. H. C. L. Escott, 72-hr canine kidney preservation without continuous perfusion. Transplantation 21:498 (1976). 38. H. Acquatella, M. M. Morales, and G. Whittembury, Ionic and histological changes in the kidney after perfusion and storage for transplantation. Use of high Naversus high K-containing solutions, Transplantation 14:480 (1972) . 39. A. H. J. Kaufman, Canine kidney preservation using a new perfusate, Lancet 2:1024 (1973) • 40.

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