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Meningococcal Vaccines. Methods and Protocols by Andrew J. Pollard, Martin C.J. Maiden

By Andrew J. Pollard, Martin C.J. Maiden

Andrew Pollard and Martin Maiden have assembled a powerful number of the newest molecular and mobile innovations for the advance, overview, and implementation of vaccines for use by contrast dreaded illness. The contributors-leading scientists, clinicians, and public well-being physicians-describe intimately the numerous ways to vaccine layout, in addition to the evaluation of immune reaction to vaccine applicants and novel vaccine formulations. well timed and entire, Meningococcal Vaccines: equipment and Protocols presents the scientist, public future health health care provider, epidemiologist, scientific microbiologist, and clinician with the basic instruments to put naked the secrets and techniques of the meningococcus, and to increase, overview, and enforce winning new meningococcal vaccines.

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273, 21,777–21,782. 7. Taha, M. , Morand, P. , and Nassif, X. (1998) Pilus-mediated adhesion of Neisseria meningitidis: the essential role of cell contact-dependent transcriptional upregulation of the PilC1 protein. Mol. Microbiol. 28, 1153–1163. 8. , Ferguson, D. , and Watt, S. M. (1996) Carcinoembryonic antigens (CD66) on epithelial cells and neutrophils are receptors for Opa proteins of pathogenic neisseriae. Mol. Microbiol. 22, 941–950. 9. , Ferguson, D. , and Moxon, E. R. (1992) Expression of the Opc protein correlates with invasion of epithelial and endothelial cells by Neisseria meningitidis.

5 (see Note 2). 2. 0 with 2 M NaOH and stir for 1 h (see Note 3). 3. , steps 3–6. 4. Perform 1HNMR spectroscopy on the product to confirm that N-propionylation is complete. 2. Oxidative Activation of N-Propionylated Serogroup B Meningococcal Polysaccharide 1. 5 (see Note 4). Keep in the dark for 30 min. 2. 1 mL 10% ethylene glycol and leave for 30 min to degrade the remaining NaIO4. N-Propionylation 59 3. , steps 3–6. Yield is 16–17 mg. 3. Conjugation of N-Propionylated Serogroup B Meningococcal Polysaccharide and TT 1.

3. 4. 5. 6. 43 solution. Dissolve 10 g Cetavlon in 80 mL dH2O and make up to 100 mL with dH2O. 3 g CaCl2 in 200 mL dH2O and make up volume to 1 L. 100% Ethanol. 85 g Na2HPO4 in dH2O and make up to 1 L. 4. 0, to 500 g phenol. Dissolve phenol completely in water bath at 50°C. 4. Purification of Polysaccharide 1. 2. 3. 4. DNAse (Sigma). RNAse A (Sigma). Proteinase (Sigma). 5 (Bio-Rad, Richmond, CA). 5. Quality Analysis 1. , Inc): 4 g resorcinol is dissolved in 100 mL deionized water. The reagent is stable for months at 4°C.

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