By W Tang, S Q Song D Thangadurai
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Preparation of cDNAs is often the first step in cloning DNA sequences of interest. Used as specific and sensitive probes in hybridization studies, because cDNAs usually do not include regulatory or other controlling sequences, and so they can be used to identify (probe) and isolate genes and their associated sequences from genomic DNA. See binary vector; carrier DNA. (2) DNA synthesised from an RNA template using reverse transcnptase. 54 CDR CDR. (complementarity-determining regions) These are regions of the variable (V) regions of light and heavy antibody chains that make contact with the antigen.
I) The two strands that constitute DNA are held together by specific hydrogen bonding between purines 34 Base substitution and pyrimidines (A pairs with T; and G pairs with C). The size of a nucleic acid molecule is often described in terms of the number of base pairs (symbol: bp) or thousand base pairs (kilobase pairs; symbol: kb; a more convenient unit) it contains. (2) A pair of complementary nitrogenous bases in a DNA molecule-adenine-thymine and guanine-cytosine. Also, the unit of measurement for DNA sequences.
However, bioconversion has several other properties, including the possibility of having several chemical steps. A major commercial application is in the manufacture of steroids. The 'basic' steroid molecule, often isolated from plants, is itself a very complicated molecule, and not one that is easy to modify by normal chemical means to produce the very specific molecules needed for drug use. However, a particular type of bioconversion that attacks only specific bits of the molecule can be used.
