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Biotechnology Methods by E. Heinzle (auth.)

By E. Heinzle (auth.)

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Other immiscible solvents like diethyl ether or ethyl acetate (EtOAc) have been used to extract selectively some AA metabolites. LTB 4 from human peripheral and rat peritoneal PMN n-a~) and from human leukocytes 15,161 have been extracted with diethyl ether from acidified media. 7 17}. 5 with 5 N HC1 and extracting with 3 volumes of diethyl ether. 2 vol. 4 with 2 N formic acid and was extracted 3 times with 1 vol. o f EtOAc. A similar procedure was used 2o}for the extraction of 15 HETE and DHETE from human eosinophil suspensions : EtOAc and diethyl ether were found equivalent.

Transport process of compounds to the membrane is important when reactions occur within the liquid boundary layer or when using thin membranes with high permeability. Non-linear behaviour of membrane penetration processes may create problems at high concentrations of volatiles. For a number of compounds connection tube material to, and temperature of the ion source have to be carefully selected. Fingerprinting of volatiles may be applicable to a number of industrial processes where process analysis is very difficult.

33) If O U R and CPR have the same value and if the outlet gas is dry we can simplifiy Eq. (33) to Af' = A d ' / ( x i n , o2/Xout. O2 -- 1). (341 Some example values are listed in Table 6. From this table it can be seen that small relative concentration differences (Ac32) will require very high precision in determination of ratios of ion currents (low values of s'd and Ad'). 1 ~ (as was claimed to be realistic 15, sos) O U R can be determined with sufficient accuracy (small enough values of s~ and Af') in almost any realistic case.

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