By W. W. Christie (Auth.)
This is often the fourth quantity of an occasional sequence of evaluation volumes facing points of lipid method. As with the 1st 3 volumes, issues were chosen which were constructing speedily lately and feature a few significance to lipid research. The authors are all prime foreign experts.
Topics coated comprise: research of plant lipoxygenase metabolites, preparative high-performance liquid chromatography of lipids, structural research of fatty acids, and research of good isotopes in lipids, between others
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Extra info for Advances in Lipid Methodology. Volume 4
An additional advantage is very intense fragment ions in their mass spectra due to cleavage vicinal to the OTMS group. Treatment of free acids with OTMS reagent affords OTMS esters that are useful to compare their mass spectra with those of OTMS-treated methyl esters. Essentially, this procedure adds m/z of 58 to all fragment ions possessing the carboxylate portion of the molecule. Thus, one can identify from which end of the chain the fragment ions originate. 30 ANALYSIS OF PLANT LIPOXYGENASE METABOLITES For OTMS formation use dry pyridine-hexamethyldisilazane-trimethylchlorosilane (2:2:3 or 2:4:2, v/v/v) usually measured in drops (for a sample of a few mg) in the order given, or a premixed solution can be used.
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196. , J. Am. Oil Chem. , 47, 352-361 (1970). 197. , Moghaddam,M. , Arch. Biochem. , 290, 436-444 (1991). 198. Galliard,T. , Biochem. , 129, 743-753 (1972). 199. Heimann,W. , Helv. Chim. Acta, 56, 463-469 (1973). 200. Esterbauer,H. , Monatsh. , 108, 963-972 (1977). Chapter2 SEPARATIONS OF MOLECULAR SPECIES OF PHOSPHOLIPIDS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Michael V. A, Scotland A. Introduction B. Principle of Molecular Species Separations by HPLC 1. Theory 2. Limitations on resolution 3.